Mechano-chemical signal conversion displays a wide-spread communication pathway for cells to translate mechano-generated signals into chemical signalling compounds which mainly occurs at the interface of membrane sites. During cell migration, geometrical changes of the cell morphology and its plasma membrane at the leading edge are essential to execute efficient protruding structures that manoeuvres the cell body persistent forward. In the case of insufficient leading edge behaviour, this can lead to detrimental consequences for the entire cellular structure in the developing and the adult organism. As curvature-dependent I-BAR domain proteins are to localise to these sites of membrane excrescence, we set out to elucidate its participation in the context of migration persistence and the following impact on the search efficiency of cell in the absence of external signals. In order to be able to investigate certain cellular structures, it necessitated the development and optimisation of specific imaging techniques such as the establishment of a micro-pattern for correlated light and electron microscopy and immuno-gold scanning electron microscopy in whole-cell samples. These two techniques helped demonstrating essential elements in the signalling cascade of mechano-chemical signal conversion and its involvement of curvature-dependent I-BAR domains.